SIM imaging resolves endocytosis of SARS-CoV-2 spike RBD in living cells
Lu Miao, Chunyu Yan, Yingzhu Chen, Wei Zhou, Xuelian Zhou, Qinglong Qiao, Zhaochao Xu
Cell Chemical Biology, doi:10.1016/j.chembiol.2023.02.001
Highlights d RBD endocytosis is resolved by imaging the location and ratio of ACE2/RBD fluorescence d Exploring the initiation and influencing factors of RBD internalization d The movement and maturation of ACE2/RBD co-localized vesicles are tracked by SIM imaging
enlightenment on the pathogenic mechanism of COVID-19 and the development of antiviral drugs, but the molecular mechanism remains to be further analyzed.
STAR+METHODS Detailed methods are provided in the online version of this paper and include the following:
AUTHOR CONTRIBUTIONS Concepts were conceived by Z.X. and L.M.; L.M. designed the experiments and analyzed the data; C.Y., X.Z., and L.M. performed live-cell experiments; W.Z. and Q.Q. performed compound synthesis; L.M. and Z.X. wrote the manuscript.
DECLARATION OF INTERESTS The authors declare no competing interests.
STAR+METHODS KEY RESOURCES
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