Chloroquine and hydroxychloroquine as ACE2 blockers to inhibit viropexis of 2019-nCoV Spike pseudotyped virus

DOI record: { "DOI": "10.1016/j.phymed.2020.153333", "ISSN": [ "0944-7113" ], "URL": "http://dx.doi.org/10.1016/j.phymed.2020.153333", "alternative-id": [ "S0944711320301653" ], "article-number": "153333", "assertion": [ { "label": "This article is maintained by", "name": "publisher", "value": "Elsevier" }, { "label": "Article Title", "name": "articletitle", "value": "Chloroquine and hydroxychloroquine as ACE2 blockers to inhibit viropexis of 2019-nCoV Spike pseudotyped virus" }, { "label": "Journal Title", "name": "journaltitle", "value": "Phytomedicine" }, { "label": "CrossRef DOI link to publisher maintained version", "name": "articlelink", "value": "https://doi.org/10.1016/j.phymed.2020.153333" }, { "label": "Content Type", "name": "content_type", "value": "article" }, { "label": "Copyright", "name": "copyright", "value": "© 2020 Elsevier GmbH. All rights reserved." } ], "author": [ { "ORCID": "http://orcid.org/0000-0002-4197-4245", "affiliation": [], "authenticated-orcid": false, "family": "Wang", "given": "Nan", "sequence": "first" }, { "affiliation": [], "family": "Han", "given": "Shengli", "sequence": "additional" }, { "affiliation": [], "family": "Liu", "given": "Rui", "sequence": "additional" }, { "affiliation": [], "family": "Meng", "given": "Liesu", "sequence": "additional" }, { "affiliation": [], "family": "He", "given": "Huaizhen", "sequence": "additional" }, { "affiliation": [], "family": "Zhang", "given": "Yongjing", "sequence": "additional" }, { "affiliation": [], "family": "Wang", "given": "Cheng", "sequence": "additional" }, { "affiliation": [], "family": "Lv", "given": "Yanni", "sequence": "additional" }, { "affiliation": [], "family": "Wang", "given": "Jue", "sequence": "additional" }, { "affiliation": [], "family": "Li", "given": "Xiaowei", "sequence": "additional" }, { "affiliation": [], "family": "Ding", "given": "Yuanyuan", "sequence": "additional" }, { "affiliation": [], "family": "Fu", "given": "Jia", "sequence": "additional" }, { "affiliation": [], "family": "Hou", "given": "Yajing", "sequence": "additional" }, { "affiliation": [], "family": "Lu", "given": "Wen", "sequence": "additional" }, { "affiliation": [], "family": "Ma", "given": "Weina", "sequence": "additional" }, { "affiliation": [], "family": "Zhan", "given": "Yingzhuan", "sequence": "additional" }, { "affiliation": [], "family": "Dai", "given": "Bingling", "sequence": "additional" }, { "affiliation": [], "family": "Zhang", "given": "Jie", "sequence": "additional" }, { "affiliation": [], "family": "Pan", "given": "Xiaoyan", "sequence": "additional" }, { "affiliation": [], "family": "Hu", "given": "Shiling", "sequence": "additional" }, { "affiliation": [], "family": "Gao", "given": "Jiapan", "sequence": "additional" }, { "affiliation": [], "family": "Jia", "given": "Qianqian", "sequence": "additional" }, { "affiliation": [], "family": "Zhang", "given": "Liyang", "sequence": "additional" }, { "affiliation": [], "family": "Ge", "given": "Shuai", "sequence": "additional" }, { "affiliation": [], "family": "Wang", "given": "Saisai", "sequence": "additional" }, { "affiliation": [], "family": "Liang", "given": "Peida", "sequence": "additional" }, { "affiliation": [], "family": "Hu", "given": "Tian", "sequence": "additional" }, { "affiliation": [], "family": "Lu", "given": "Jiayu", "sequence": "additional" }, { "affiliation": [], "family": "Wang", "given": "Xiangjun", "sequence": "additional" }, { "affiliation": [], "family": "Zhou", "given": "Huaxin", "sequence": "additional" }, { "affiliation": [], "family": "Ta", "given": "Wenjing", "sequence": "additional" }, { "affiliation": [], "family": "Wang", "given": "Yuejin", "sequence": "additional" }, { "affiliation": [], "family": "Lu", "given": "Shemin", "sequence": "additional" }, { "ORCID": "http://orcid.org/0000-0002-0880-7904", "affiliation": [], "authenticated-orcid": false, "family": "He", "given": "Langchong", "sequence": "additional" } ], "container-title": "Phytomedicine", "container-title-short": "Phytomedicine", "content-domain": { "crossmark-restriction": true, "domain": [ "elsevier.com", "sciencedirect.com" ] }, "created": { "date-parts": [ [ 2020, 9, 2 ] ], "date-time": "2020-09-02T16:28:21Z", "timestamp": 1599064101000 }, "deposited": { "date-parts": [ [ 2020, 10, 19 ] ], "date-time": "2020-10-19T06:10:08Z", "timestamp": 1603087808000 }, "funder": [ { "DOI": "10.13039/501100001809", "award": [ "81930096" ], "doi-asserted-by": "publisher", "name": "National Natural Science Foundation of China" }, { "DOI": "10.13039/501100012429", "award": [ "xzy032020042" ], "doi-asserted-by": "publisher", "name": "Central Universities" } ], "indexed": { "date-parts": [ [ 2024, 4, 26 ] ], "date-time": "2024-04-26T21:08:25Z", "timestamp": 1714165705608 }, "is-referenced-by-count": 43, "issued": { "date-parts": [ [ 2020, 12 ] ] }, "language": "en", "license": [ { "URL": "https://www.elsevier.com/tdm/userlicense/1.0/", "content-version": "tdm", "delay-in-days": 0, "start": { "date-parts": [ [ 2020, 12, 1 ] ], "date-time": "2020-12-01T00:00:00Z", "timestamp": 1606780800000 } } ], "link": [ { "URL": "https://api.elsevier.com/content/article/PII:S0944711320301653?httpAccept=text/xml", "content-type": "text/xml", "content-version": "vor", "intended-application": "text-mining" }, { "URL": "https://api.elsevier.com/content/article/PII:S0944711320301653?httpAccept=text/plain", "content-type": "text/plain", "content-version": "vor", "intended-application": "text-mining" } ], "member": "78", "original-title": [], "page": "153333", "prefix": "10.1016", "published": { "date-parts": [ [ 2020, 12 ] ] }, "published-print": { "date-parts": [ [ 2020, 12 ] ] }, "publisher": "Elsevier BV", "reference": [ { "DOI": "10.1080/07391102.2020.1763199", "article-title": "In silico study the inhibition of angiotensin converting enzyme 2 receptor of COVID-19 by ammoides verticillata components harvested from Western Algeria", "author": "Abdelli", "doi-asserted-by": "crossref", "journal-title": "J. Biomol. Struct. Dyn.", "key": "10.1016/j.phymed.2020.153333_bib0001", "year": "2020" }, { "DOI": "10.1002/prp2.293", "article-title": "Targeting endosomal acidification by chloroquine analogs as a promising strategy for the treatment of emerging viral diseases", "author": "Al-Bari", "doi-asserted-by": "crossref", "journal-title": "Pharmacol. Res. Perspe.", "key": "10.1016/j.phymed.2020.153333_bib0002", "volume": "5", "year": "2017" }, { "DOI": "10.1002/jmv.25887", "article-title": "Hyperglycemia, hydroxychloroquine, and the COVID-19 pandemic", "author": "Brufsky", "doi-asserted-by": "crossref", "first-page": "770", "journal-title": "J. Med. Virol.", "key": "10.1016/j.phymed.2020.153333_bib0003", "volume": "92", "year": "2020" }, { "DOI": "10.3390/v8120322", "article-title": "Chloroquine, an endocytosis blocking agent, inhibits zika virus infection in different cell models", "author": "Delvecchio", "doi-asserted-by": "crossref", "journal-title": "Viruses", "key": "10.1016/j.phymed.2020.153333_bib0004", "volume": "8", "year": "2016" }, { "DOI": "10.1016/j.ijantimicag.2020.105960", "article-title": "Structural and molecular modelling studies reveal a new mechanism of action of chloroquine and hydroxychloroquine against SARS-CoV-2 infection", "author": "Fantini", "doi-asserted-by": "crossref", "journal-title": "Int. J. Antimicrob. Agents", "key": "10.1016/j.phymed.2020.153333_bib0005", "volume": "55", "year": "2020" }, { "DOI": "10.1136/bmj.m1432", "article-title": "Chloroquine and hydroxychloroquine in covid-19", "author": "Ferner", "doi-asserted-by": "crossref", "first-page": "m1432", "journal-title": "BMJ", "key": "10.1016/j.phymed.2020.153333_bib0006", "volume": "369", "year": "2020" }, { "DOI": "10.5582/bst.2020.01047", "article-title": "Breakthrough: chloroquine phosphate has shown apparent efficacy in treatment of COVID-19 associated pneumonia in clinical studies", "author": "Gao", "doi-asserted-by": "crossref", "first-page": "72", "journal-title": "Biosci. Trends", "key": "10.1016/j.phymed.2020.153333_bib0007", "volume": "14", "year": "2020" }, { "DOI": "10.1016/S0140-6736(95)92083-8", "article-title": "Chloroquine-resistant falciparum malaria in Mauritania", "author": "Gasquet", "doi-asserted-by": "crossref", "first-page": "1556", "journal-title": "Lancet", "key": "10.1016/j.phymed.2020.153333_bib0008", "volume": "346", "year": "1995" }, { "DOI": "10.1016/j.ijantimicag.2020.105949", "article-title": "Hydroxychloroquine and azithromycin as a treatment of COVID-19: results of an open-label non-randomized clinical trial", "author": "Gautret", "doi-asserted-by": "crossref", "journal-title": "Int. J. Antimicrob. Agents", "key": "10.1016/j.phymed.2020.153333_bib0009", "year": "2020" }, { "DOI": "10.1056/NEJMoa2012410", "article-title": "Observational study of hydroxychloroquine in hospitalized patients with Covid-19", "author": "Geleris", "doi-asserted-by": "crossref", "first-page": "2411", "journal-title": "N. Engl. J. Med.", "key": "10.1016/j.phymed.2020.153333_bib0010", "volume": "382", "year": "2020" }, { "DOI": "10.1016/S1473-3099(16)30378-4", "article-title": "Safety, tolerability, and efficacy of repeated doses of dihydroartemisinin-piperaquine for prevention and treatment of malaria: a systematic review and meta-analysis", "author": "Gutman", "doi-asserted-by": "crossref", "first-page": "184", "journal-title": "Lancet Infect. Dis.", "key": "10.1016/j.phymed.2020.153333_bib0011", "volume": "17", "year": "2017" }, { "DOI": "10.1016/j.cell.2020.02.052", "article-title": "SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor", "author": "Hoffmann", "doi-asserted-by": "crossref", "first-page": "271", "journal-title": "Cell", "key": "10.1016/j.phymed.2020.153333_bib0012", "volume": "181", "year": "2020" }, { "DOI": "10.1016/j.bbrc.2004.08.085", "article-title": "In vitro inhibition of severe acute respiratory syndrome coronavirus by chloroquine", "author": "Keyaerts", "doi-asserted-by": "crossref", "first-page": "264", "journal-title": "Biochem. Biophys. Res. Commun.", "key": "10.1016/j.phymed.2020.153333_bib0013", "volume": "323", "year": "2004" }, { "DOI": "10.1016/j.mehy.2020.109783", "article-title": "Hydroxychloroquine as an aerosol might markedly reduce and even prevent severe clinical symptoms after SARS-CoV-2 infection", "author": "Klimke", "doi-asserted-by": "crossref", "journal-title": "Med. Hypotheses", "key": "10.1016/j.phymed.2020.153333_bib0014", "volume": "142", "year": "2020" }, { "DOI": "10.1038/s41467-020-16048-4", "article-title": "Neutralization of SARS-CoV-2 spike pseudotyped virus by recombinant ACE2-Ig", "author": "Lei", "doi-asserted-by": "crossref", "first-page": "2070", "journal-title": "Nat. Commun.", "key": "10.1016/j.phymed.2020.153333_bib0015", "volume": "11", "year": "2020" }, { "DOI": "10.1080/15548627.2018.1474314", "article-title": "Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion", "author": "Mauthe", "doi-asserted-by": "crossref", "first-page": "1435", "journal-title": "Autophagy", "key": "10.1016/j.phymed.2020.153333_bib0016", "volume": "14", "year": "2018" }, { "article-title": "Efficacy of chloroquine and hydroxychloroquine in the treatment of COVID-19", "author": "Meo", "first-page": "4539", "journal-title": "Eur. Rev. Med. Pharmacol. Sci.", "key": "10.1016/j.phymed.2020.153333_bib0017", "volume": "24", "year": "2020" }, { "DOI": "10.1001/jamacardio.2020.1834", "article-title": "Risk of QT interval prolongation associated with use of hydroxychloroquine with or without concomitant azithromycin among hospitalized patients testing positive for coronavirus disease 2019 (COVID-19)", "author": "Mercuro", "doi-asserted-by": "crossref", "journal-title": "JAMA Cardiol.", "key": "10.1016/j.phymed.2020.153333_bib0018", "year": "2020" }, { "article-title": "Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19", "author": "Mingxing", "journal-title": "Natl. Sci. Rev.", "key": "10.1016/j.phymed.2020.153333_bib0019", "year": "2020" }, { "DOI": "10.1016/j.medmal.2020.03.006", "article-title": "No evidence of rapid antiviral clearance or clinical benefit with the combination of hydroxychloroquine and azithromycin in patients with severe COVID-19 infection", "author": "Molina", "doi-asserted-by": "crossref", "first-page": "384", "journal-title": "Med. Mal. Infect.", "key": "10.1016/j.phymed.2020.153333_bib0020", "volume": "50", "year": "2020" }, { "DOI": "10.1080/22221751.2020.1743767", "article-title": "Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2", "author": "Nie", "doi-asserted-by": "crossref", "first-page": "680", "journal-title": "Emerg. Microbes Infec.", "key": "10.1016/j.phymed.2020.153333_bib0021", "volume": "9", "year": "2020" }, { "DOI": "10.1038/s41467-020-15562-9", "article-title": "Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV", "author": "Ou", "doi-asserted-by": "crossref", "journal-title": "Nat. Commun.", "key": "10.1016/j.phymed.2020.153333_bib0022", "volume": "11", "year": "2020" }, { "DOI": "10.1007/s40261-018-0656-y", "article-title": "Current and future use of chloroquine and hydroxychloroquine in infectious, immune, neoplastic, and neurological diseases: a mini-review", "author": "Plantone", "doi-asserted-by": "crossref", "first-page": "653", "journal-title": "Clin. Drug. Investig.", "key": "10.1016/j.phymed.2020.153333_bib0023", "volume": "38", "year": "2018" }, { "DOI": "10.1007/s10787-015-0239-y", "article-title": "Therapy and pharmacological properties of hydroxychloroquine and chloroquine in treatment of systemic lupus erythematosus, rheumatoid arthritis and related diseases", "author": "Rainsford", "doi-asserted-by": "crossref", "first-page": "231", "journal-title": "Inflammopharmacology", "key": "10.1016/j.phymed.2020.153333_bib0024", "volume": "23", "year": "2015" }, { "DOI": "10.1016/S1473-3099(11)70092-5", "article-title": "Use of chloroquine in viral diseases", "author": "Savarino", "doi-asserted-by": "crossref", "first-page": "653", "journal-title": "Lancet Infect. Dis.", "key": "10.1016/j.phymed.2020.153333_bib0025", "volume": "11", "year": "2011" }, { "DOI": "10.1016/S1473-3099(06)70361-9", "article-title": "New insights into the antiviral effects of chloroquine", "author": "Savarino", "doi-asserted-by": "crossref", "first-page": "67", "journal-title": "Lancet Infect. Dis.", "key": "10.1016/j.phymed.2020.153333_bib0026", "volume": "6", "year": "2006" }, { "DOI": "10.1038/s41584-020-0372-x", "article-title": "Mechanisms of action of hydroxychloroquine and chloroquine: implications for rheumatology", "author": "Schrezenmeier", "doi-asserted-by": "crossref", "first-page": "155", "journal-title": "Nat. Rev. Rheumatol.", "key": "10.1016/j.phymed.2020.153333_bib0027", "volume": "16", "year": "2020" }, { "DOI": "10.1073/pnas.2003138117", "article-title": "Cell entry mechanisms of SARS-CoV-2", "author": "Shang", "doi-asserted-by": "crossref", "first-page": "11727", "journal-title": "P. Natl. Acad. Sci. USA", "key": "10.1016/j.phymed.2020.153333_bib0028", "volume": "117", "year": "2020" }, { "DOI": "10.1186/1743-422X-2-69", "article-title": "Chloroquine is a potent inhibitor of SARS coronavirus infection and spread", "author": "Vincent", "doi-asserted-by": "crossref", "first-page": "69", "journal-title": "Virol. J.", "key": "10.1016/j.phymed.2020.153333_bib0029", "volume": "2", "year": "2005" }, { "DOI": "10.1016/S0140-6736(20)30185-9", "article-title": "A novel coronavirus outbreak of global health concern", "author": "Wang", "doi-asserted-by": "crossref", "first-page": "470", "journal-title": "Lancet", "key": "10.1016/j.phymed.2020.153333_bib0030", "volume": "395", "year": "2020" }, { "DOI": "10.1038/s41422-020-0282-0", "article-title": "Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro", "author": "Wang", "doi-asserted-by": "crossref", "first-page": "269", "journal-title": "Cell Res.", "key": "10.1016/j.phymed.2020.153333_bib0031", "volume": "30", "year": "2020" }, { "DOI": "10.1056/NEJM199609123351107", "article-title": "The treatment of malaria", "author": "White", "doi-asserted-by": "crossref", "first-page": "800", "journal-title": "N. Engl. J. Med.", "key": "10.1016/j.phymed.2020.153333_bib0032", "volume": "335", "year": "1996" }, { "DOI": "10.1016/S1473-3099(07)70187-1", "article-title": "Cardiotoxicity of antimalarial drugs", "author": "White", "doi-asserted-by": "crossref", "first-page": "549", "journal-title": "Lancet Infect. Dis.", "key": "10.1016/j.phymed.2020.153333_bib0033", "volume": "7", "year": "2007" }, { "DOI": "10.1016/S0140-6736(13)60024-0", "article-title": "Malaria", "author": "White", "doi-asserted-by": "crossref", "first-page": "723", "journal-title": "Lancet", "key": "10.1016/j.phymed.2020.153333_bib0034", "volume": "383", "year": "2014" }, { "DOI": "10.1126/science.abb2507", "article-title": "Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation", "author": "Wrapp", "doi-asserted-by": "crossref", "first-page": "1260", "journal-title": "Science", "key": "10.1016/j.phymed.2020.153333_bib0035", "volume": "367", "year": "2020" }, { "DOI": "10.1126/science.abc2241", "article-title": "A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2", "author": "Wu", "doi-asserted-by": "crossref", "first-page": "1274", "journal-title": "Science", "key": "10.1016/j.phymed.2020.153333_bib0036", "volume": "368", "year": "2020" }, { "DOI": "10.3390/v12050491", "article-title": "ACE2: the key molecule for understanding the pathophysiology of severe and critical conditions of COVID-19: demon or angel?", "author": "Xiao", "doi-asserted-by": "crossref", "journal-title": "Viruses", "key": "10.1016/j.phymed.2020.153333_bib0037", "volume": "12", "year": "2020" }, { "DOI": "10.1126/science.abb2762", "article-title": "Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2", "author": "Yan", "doi-asserted-by": "crossref", "first-page": "1444", "journal-title": "Science", "key": "10.1016/j.phymed.2020.153333_bib0038", "volume": "367", "year": "2020" }, { "DOI": "10.1038/s41569-020-0360-5", "article-title": "COVID-19 and the cardiovascular system", "author": "Zheng", "doi-asserted-by": "crossref", "first-page": "259", "journal-title": "Nat. Rev. Cardiol.", "key": "10.1016/j.phymed.2020.153333_bib0039", "volume": "17", "year": "2020" }, { "DOI": "10.1007/s11684-020-0754-0", "article-title": "Single-cell RNA-seq data analysis on the receptor ACE2 expression reveals the potential risk of different human organs vulnerable to 2019-nCoV infection", "author": "Zou", "doi-asserted-by": "crossref", "first-page": "185", "journal-title": "Front. Med.", "key": "10.1016/j.phymed.2020.153333_bib0040", "volume": "14", "year": "2020" } ], "reference-count": 40, "references-count": 40, "relation": { "has-preprint": [ { "asserted-by": "object", "id": "10.1101/2020.06.22.164665", "id-type": "doi" } ] }, "resource": { "primary": { "URL": "https://linkinghub.elsevier.com/retrieve/pii/S0944711320301653" } }, "score": 1, "short-title": [], "source": "Crossref", "subject": [], "subtitle": [], "title": "Chloroquine and hydroxychloroquine as ACE2 blockers to inhibit viropexis of 2019-nCoV Spike pseudotyped virus", "type": "journal-article", "update-policy": "http://dx.doi.org/10.1016/elsevier_cm_policy", "volume": "79" }