Pre-Infection Innate Immunity Attenuates SARS-CoV-2 Infection and Viral Load in iPSC-Derived Alveolar Epithelial Type 2 Cells
Satish Kumar, Jose Granados, Miriam Aceves, Juan Peralta, Ana C Leandro, John Thomas, Sarah Williams-Blangero, Joanne E Curran, John Blangero
Cells, doi:10.3390/cells13050369
A large portion of the heterogeneity in coronavirus disease 2019 (COVID-19) susceptibility and severity of illness (SOI) remains poorly understood. Recent evidence suggests that SARS-CoV-2 infection-associated damage to alveolar epithelial type 2 cells (AT2s) in the distal lung may directly contribute to disease severity and poor prognosis in COVID-19 patients. Our in vitro modeling of SARS-CoV-2 infection in induced pluripotent stem cell (iPSC)-derived AT2s from 10 different individuals showed interindividual variability in infection susceptibility and the postinfection cellular viral load. To understand the underlying mechanism of the AT2 ′ s capacity to regulate SARS-CoV-2 infection and cellular viral load, a genome-wide differential gene expression analysis between the mock and SARS-CoV-2 infection-challenged AT2s was performed. The 1393 genes, which were significantly (one-way ANOVA FDR-corrected p ≤ 0.05; FC abs ≥ 2.0) differentially expressed (DE), suggest significant upregulation of viral infection-related cellular innate immune response pathways (p-value ≤ 0.05; activation z-score ≥ 3.5), and significant downregulation of the cholesterol-and xenobiotic-related metabolic pathways (p-value ≤ 0.05; activation z-score ≤ -3.5). Whilst the effect of post-SARS-CoV-2 infection response on the infection susceptibility and postinfection viral load in AT2s is not clear, interestingly, pre-infection (mock-challenged) expression of 238 DE genes showed a high correlation with the postinfection SARS-CoV-2 viral load (FDR-corrected p-value ≤ 0.05 and r 2absolute ≥ 0.57). The 85 genes whose expression was negatively correlated with the viral load showed significant enrichment in viral recognition and cytokine-mediated innate immune GO biological processes (p-value range: 4.65 × 10 -10 to 2.24 × 10 -6 ). The 153 genes whose expression was positively correlated with the viral load showed significant enrichment in cholesterol homeostasis, extracellular matrix, and MAPK/ERK pathway-related GO biological processes (p-value range: 5.06 × 10 -5 to 6.53 × 10 -4 ). Overall, our results strongly suggest that AT2s' pre-infection innate immunity and metabolic state affect their susceptibility to SARS-CoV-2 infection and viral load.
Conflicts of Interest: The author declares no conflicts of interest.
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'abstract': '<jats:p>A large portion of the heterogeneity in coronavirus disease 2019 (COVID-19) '
'susceptibility and severity of illness (SOI) remains poorly understood. Recent evidence '
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'COVID-19 patients. Our in vitro modeling of SARS-CoV-2 infection in induced pluripotent stem '
'cell (iPSC)-derived AT2s from 10 different individuals showed interindividual variability in '
'infection susceptibility and the postinfection cellular viral load. To understand the '
'underlying mechanism of the AT2′s capacity to regulate SARS-CoV-2 infection and cellular '
'viral load, a genome-wide differential gene expression analysis between the mock and '
'SARS-CoV-2 infection-challenged AT2s was performed. The 1393 genes, which were significantly '
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'significant upregulation of viral infection-related cellular innate immune response pathways '
'(p-value ≤ 0.05; activation z-score ≥ 3.5), and significant downregulation of the '
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'susceptibility and postinfection viral load in AT2s is not clear, interestingly, '
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