Getting in on the action: New tools to see SARS-CoV-2 infect a cell
Scott B Hansen, Zixuan Yuan
Cell Chemical Biology, doi:10.1016/j.chembiol.2023.02.010
In this issue of Cell Chemical Biology, Miao et al. develop probes for live cell tracking of SARS-CoV-2. The probes reveal the endocytic pathway for viral entry. Unexpectedly, the antiviral compound BafA1 traps the virus on the cell surface, highlighting the power of super-resolution imaging in live cells.
References
Bayati, Kumar, Francis, Mcpherson, SARS-CoV-2 infects cells after viral entry via clathrin-mediated endocytosis, J. Biol. Chem,
doi:10.1016/j.jbc.2021.100306
Bonazzi, Hennezel, Beckwith, Xu, Fazal et al., Discovery and characterization of a selective IKZF2 glue degrader for cancer immunotherapy, Cell Chemical Biology,
doi:10.1016/j.chembiol.2023.02.005
Fischer, Bohm, Lydeard, Yang, Stadler et al., Structure of the DDB1-CRBN E3 ubiq-uitin ligase in complex with thalidomide, Nature,
doi:10.1038/nature13527
Hoffmann, Kleine-Weber, Schroeder, Kr€ Uger, Herrler et al., SARS-CoV-2 cell entry Depends on ACE2 and TMPRSS2 and is blocked by a Clinically Proven Protease inhibitor, Cell,
doi:10.1016/j.cell.2020.02.052
Kim, Barnitz, Kreslavsky, Brown, Moffett et al., Stable inhibitory activity of regulatory T cells requires the transcription factor Helios, Science,
doi:10.1126/science.aad0616
Kreutzberger, Sanyal, Saminathan, Bloyet, Stumpf et al., SARS-CoV-2 requires acidic pH to infect cells, Proc. Natl. Acad. Sci. USA,
doi:10.1073/pnas.2209514119
Kronke, Udeshi, Narla, Grauman, Hurst et al., Lenalidomide causes selective degradation of IKZF1 and IKZF3 in multiple myeloma cells, Science,
doi:10.1126/science.1244851
Lu, Middleton, Sun, Naniong, Ott et al., The myeloma drug lenalidomide promotes the cereblon-dependent destruction of Ikaros proteins, Science,
doi:10.1126/science.1244917
Matyskiela, Lu, Ito, Pagarigan, Lu et al., A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase, Nature,
doi:10.1038/nature18611
Meng, Abdullahi, Ferreira, Goonawardane, Saito et al., Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts infectivity and fusogenicity, Nature,
doi:10.1038/s41586-022-04474-x
Miao, Yan, Chen, Zhou, Zhou et al., SIM imaging Resolves endocytosis of SARS-CoV-2 spike RBD in living cells, Cell Chem Biol
Nakagawa, Sido, Reyes, Kiers, Cantor et al., Instability of Helios-deficient Tregs is associated with conversion to a T-effector phenotype and enhanced antitumor immunity, Proc. Natl. Acad. Sci. USA,
doi:10.1073/pnas.1604765113
Rusinova, He, Andersen, Mechanisms underlying drug-mediated regulation of membrane protein function, Proc. Natl. Acad. Sci. USA,
doi:10.1073/pnas.2113229118
Sievers, Petzold, Bunker, Renneville, S1abicki et al., Defining the human C2H2 zinc finger degrome targeted by thalidomide analogs through CRBN, Science,
doi:10.1126/science.aat0572
Wang, Verano, Nowak, Yuan, Donovan et al., Acute pharmacological degradation of Helios destabilizes regulatory T cells, Nat. Chem. Biol,
doi:10.1038/s41589-021-00802-w
Yoshimori, Yamamoto, Moriyama, Futai, Tashiro, Bafilomycin A1, a specific inhibitor of vacuolar-type H+-ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells, J. Biol. Chem,
doi:10.1016/s0021-9258(19)47429-2
Yuan, Hansen, Cholesterol regulation of membrane proteins revealed by two-color super-resolution imaging, Membranes,
doi:10.3390/membranes13020250
Yuan, Pavel, Wang, Kwachukwu, Mediouni et al., Hydroxychloroquine blocks SARS-CoV-2 entry into the endocytic pathway in mammalian cell culture, Commun. Biol,
doi:10.1038/s42003-022-03841-8
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